Quantify Secreted T Cell Activation Markers

Although Chimeric Antigen Receptor (CAR) T cells have great therapeutic utility, the manufacturing workflow is complex and stringent regulatory requirements must be met. CAR T cells must be thoroughly characterized in vitro before infusion into the patient. Bio-Techne is pleased to offer Simple Plex T Cell activation marker assays to support cell therapy development and manufacturing scientists. Simple Plex assays on the Ella platform offer a rapid and precise assessment of secreted T cell activation markers to boost cell therapy development and manufacturing efficiency.

In this application note, we use TcBuster™, Bio-Techne’s novel non-viral gene delivery technology, to generate CD19 CAR T cells and validate the use of Ella as a T Cell activation marker assay platform. Read on to learn more.