Quantification And Qualification Of Adeno-Associated Virus (AAV) Using Dedicated CGT Assays And The QIAcuity® Digital PCR System

By Domenica Martorana, Jonathan Thorn, Mario Quinto, Anne Schieren, Colin Donohoe, Francesca Di Pasquale, Özlem Karalay, Ronny Kellner, Andreas Missel, Andreas Hecker, and Daniel Heinz Löfgren, QIAGEN

Quantitative PCR (qPCR) is commonly used for AAV quantification, but it relies on reference standards and can show variability across operators. To overcome these limitations, the QIAcuity® Digital PCR System with dedicated CGT assays delivers highly accurate, reproducible vector genome titration with a workflow as simple as qPCR.

These dPCR assays enable both singleplex and multiplex analysis of AAV genomes, supporting quantification and qualification without standard curves. With broad dynamic range, low variation between users, and assays tailored for CGT, QIAcuity improves consistency and confidence in AAV analytics.

Access the full poster to explore how QIAcuity can streamline your AAV quantification workflow.