Quality Control In The PacBio HiFi Whole Genome Sequencing Library Preparation Workflow

By Aurelie Souppe, Gloria Diaz, and Greg Young, PacBio, and Timothy Butler and Whitney Pike, Agilent Technologies, Inc.

Choosing the right electrophoresis system can make or break DNA quality assessment in long‑read sequencing. This application note lays out a straightforward comparison of key platforms, showing how sizing precision, resolution, and sensitivity directly influence the accuracy of high‑molecular‑weight DNA evaluation. It walks through practical examples of common bottlenecks—such as detecting degradation, confirming fragment length distributions, and validating extraction consistency—and clarifies which systems provide the clarity needed at each QC checkpoint. Gain a grounded perspective on what truly matters in long‑read workflows, from early sample acceptance through library prep validation.

Access the full asset to explore the detailed comparisons and workflow examples.