Purification Of Antibody Fragments And Single Domain Antibodies Using Protein A Chromatography

Besides the use for Fc containing antibodies and constructs, the Protein A resin Amsphere™ A3 can be used for the purification of antibody fragment formats, like the VHH single domain antibodies (sdAbs), constructs containing a VH3 domain like Fragment Antigen-Binding molecules (Fabs) and different fusion proteins such as single-chain variable fragment (scFvs).

This application note contains key information regarding the use of Amsphere™ A3 for the affinity purification of these VHH sdAbs and antibody fragments that contain a VH domain, but not an Fc region. Recommendations for experimental protocols are given. The Amsphere™ A3 Instruction Manual provides more comprehensive information on the general use of this resin.