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Is your protein unable to bind efficiently to your resin? If you are performing flow-through (FT) purification, monomer recovery could be poor in the flow-through fraction due to nonoptimal conditions, a nonideal resin, or both.
Performing a design of experiment (DOE) study of different pH, salt concentrations, and resins will help you select the best conditions and resin for your purification success.
Read how Nuvia aPrime 4A Resin’s superior performance is the result of its optimized pore structures and surface properties, which offer efficient mass transfer and minimal nonspecific interaction between biomolecules and this chromatography media