Protein Fluorescence Analyzer

CST’s Protein Fluorescence Analyzer contains a PX2+ UV Photometer that uses optical filters to provide specific excitation/emission wavelength ranges chosen to coincide with protein fluorescence. Fluorescence occurs when a molecule absorbs light energy at one wavelength and re-emits light at another, typically longer, wavelength. The wavelength where the maximum absorption occurs is called the excitation wavelength, and the wavelength where the maximum emission occurs is called the emission wavelength.

The Protein Fluorescence Analyzer monitors intrinsic protein fluorescence by exciting protein typically at 280 nm and measuring the emission at approximately 350 nm. Light emitted by the protein sample correlates to the concentration of protein present. Fluorescence measurements are highly sensitive and are more suitable for low protein concentrations as compared to absorbance measurements. Monitoring protein concentrations by measuring their intrinsic fluorescence is an excellent method because it is rapid, simple, and requires no expensive dyes or labeling.