Case Study

Protein A Resin Reduction Achieved With Process Transfer From Batch To BioSMB

Source: Sartorius
Sartorius PD_Line

Customer Challenge

Sanofi’s key driver for this study was to reduce the operating costs of existing purification steps with high resin costs. Regulatory concerns were to be tested by determining product quality consistency through process transfer and scale-up. The feasibility of extended purifications at production scale was also determined by evaluating BioSMB system robustness.


The aim of this study was to explore the benefits of multi-column chromatography and application to a perfusion based process. Resin savings and the capability of continuous chromatography to run for long process times were important decision factors in the selection of the technology.

This case study centred on transferring an existing batch chromatography process into a multi column format and then scaling up that process to an continuous perfusion run. The bioreactor was a 50 L single-use perfusion setup where the maintenance of aseptic conditions and flow path robustness were key to meet the target of a 10 day run time that ultimately processed a feedstock volume of approximately 800 L.

access the Case Study!

Get unlimited access to:

Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue. X

Enter your credentials below to log in. Not yet a member of Bioprocess Online? Subscribe today.

Subscribe to Bioprocess Online X

Please enter your email address and create a password to access the full content, Or log in to your account to continue.


Subscribe to Bioprocess Online