Proficiency Testing
Source: StemCell Technologies
Standardized hematopoietic colony assay to reduce the variation in colony schoring among participants.
StemCell Technologies is committed to standardizing hematopoietic colony assays. Despite the advent of standardized media (eg. MethoCult), significant variability in assay results performed by individuals still hinders the ability to directly compare data generated at different centers. This variability has been associated with both sample preparation and differences in colony identification and enumeration. In order to address the issues arising from the variability encountered in these two areas, StemCell designed a two-step proficiency testing approach. In addition to colony identification and enumeration, the program incorporates upstream events including cell thawing, dilution, inoculation and plating. This new program runs bi-annually and maintains the anonymity of all participants. We believe that this proficiency testing program will reduce the variability associated with colony assays and assist individuals to identify specific areas that might benefit from continuing education and training.