Protein A chromatography capture steps typically use 20-cm bed heights. This can often lead to longer processing times and reduces cost-effectiveness, particularly in clinical trials where the full capacity of the resin is not utilized. In many instances, this may be the reason for higher overall downstream processing costs and disproportionately higher costs of the Protein A affinity capture step.
Explore innovative strategies and practical applications to increase resin utility, resulting in improvements in costs and time efficiencies in Protein A affinity capture.
Key Learning Objectives:
- Understand key characteristics of Protein A affinity resins and their impact on process performance.
- Understand constraints and limitations of common chromatography resins and how these can be overcome.
- View practical models for downstream process intensification.
- Understand how to maximize cost-efficiencies and productivity with reduced buffer consumption and increased resin utilization.