Principles And Benefits Of Process Intensification For Affinity Chromatography

During a Q&A session, Purolite Field Application Specialist, William Bowen, PhD, discussed process intensification strategies, including SMART Cycling and Purolite’s patented Jetting technology, used by customers to improve downstream processing. SMART Cycling enables cost savings and productivity improvements without the need for facility changes or new equipment, unlike continuous chromatography. The choice between SMART Cycling or continuous chromatography depends on a facility's limitations and resources. Bowen noted that reducing bed height is an efficient process intensification strategy that can lead to consistent packs. He stated that SMART Cycling's outcomes depend on the strategy applied, with the main goal being to increase resin utility by reducing its volume. He mentioned Purolite’s Praesto® A50 resins and jetted beads are used in continuous applications. Bowen concluded by recommending the use of 0.1 molar sodium hydroxide and advising against using 0.5 molar caustic in every cycle.