Primer Premier 5 Design Program

Source: Premier Biosoft International
A fully featured, competitively priced primer design program to design specific, sensitive, high yield primers
Primer Premier 5: A fully featured, competitively priced primer design program to design specific, sensitive, high yield primers
  • Cross species primers - design primers for amplifying sequences from multiple species
  • Pathogenic detection primers - design primers in highly conserved regions
  • Allele specific primers
  • - design primers to exclusively amplify a single member of a set of related sequences
  • Degenerate primers - start with an amino acid sequence, reverse translate and design primers in regions of low degeneracy automatically
  • Nested/multiplex primers
  • - select primers for nested PCR reaction by checking multiple primer homology among candidate primers
  • Restriction enzyme and motif analysis - select from the database of over 800 enzymes and 200 common motifs
  • ClustalW alignments
  • - load the aligned sequences after performing Clustal W alignment using any resource, including EMBL
  • Multiple sequence alignment - use the popular Clustal W algorithm to align sequences from your desktop (available in Windows version only)
  • Alignment editor – manually insert/delete/move gaps in an alignment
  • Long PCR – handles sequences up to 50 KB

Primer 5 is the first program to fully integrate multiple sequence alignment (ClustalW) with primer design to facilitate the design of primers that can be used for cross species, pathogenic or allele specific research.

PRIMER PREMIER is one of the most comprehensive primer design tools available to design optimal primers for PCR, sequencing and hybridization quickly. Primers can be designed automatically or manually. With the automatic search, the program gradually relaxes the stringency of the search until the best possible primers available within the target sequence are found. The primers are located to optimize for Tm, GC%, GC clamping, 3' end stability, and to minimize hairpins and dimers. For experimental designs requiring a finer degree of control, the manual search allows exact primer criteria to be set and all primers meeting the criteria are located. The resultant primers are ranked both individually and paired based on their priming efficiency. The selected primers or pairs can be ordered easily by printing the synthesis order form provided.

Premier Biosoft International, 3786 Corina Way, Palo Alto, CA 94303-4504. Tel: 650-856-2703; Fax: 650-843-1250.