Preparative Anion Exchange Chromatography For The Purification Of Phosphorothioate DNA Oligonucleotides
By Anton Posch, Franziska Kollmann, Joseph Poblete, Sharon Bola, Khaled Mriziq, and Elizabeth Dreskin
![DNA-GettyImages-1407359737 DNA-GettyImages-1407359737](https://vertassets.blob.core.windows.net/image/8f0e9412/8f0e9412-276c-4328-98e8-5281b09939cd/375_250-dna_gettyimages_1407359737.jpg)
Incomplete or unusual sequences of oligonucleotide (ON) during solid-phase synthesis are key impurities that need to be removed before these molecules can be used as therapeutics. The production of pure therapeutic DNA ONs requires efficient, scalable, and cost-effective chromatographic purification methods applied after chemical synthesis.
This study explores how preparative AEX chromatography with Nuvia HP-Q Resin compares to the typical chromatography utilized in removing length-based impurities. Key process parameters such as flow rate, gradient slope, buffer pH, and dynamic binding capacity (DBC) were factors in the testing. Discover if the AEX Nuvia HP-Q Resin is well-suited for the purification of standard ONs with phosphodiester backbones.
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