By Kayla Barry, Jonathan Rheuben, Shaoyang Anthony Yeh, Andrew Stewart, Luisa Jaime Ladron de Guevara, Keyvan Dastmalchi, Sophia Bringas, Joshua Wine, Alexander Fortner, Kellen Dragan
Bispecific antibodies (bsAbs) can become unstable under typical Protein-A elution conditions (pH 3—3.5), causing aggregation and fragmentation. Novel Purolite Praesto Jetted A50 HipH resin is designed to elute antibodies in a higher pH range (up to pH 5). The unique properties of this resin allow for efficient elution at milder pH levels to conventional protein A chromatography. Two bsAb molecules were evaluated by Praesto Jetted A50 HipH and a Standard Protein A resin to maximize monomer purity. Praesto Jetted A50 HipH resin demonstrated increased separation and monomeric recovery when compared to the standard resin.