Product/Service

Praesto® Jetted A50 HipH For MAb Purification

Antibody Purification And Immunoprecipitation

Praesto®  Jetted A50 HipH is a commercially available Protein A resin designed specifically for elution of Fc-containing molecules at higher pH levels.

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Praesto Jetted A50 HipH is manufactured using our patented Jetting technology, which produces consistent agarose beads with a uniform particle size distribution.

Principal Applications

  • MAb Purification

Advantages

  • Manufactured using patented Jetting technique
  • Uniform particle size
  • Repligen NGL HipH (NGLU)
  • Dynamic binding capacity up to ~60 mg/hIgG/ml resin
  • Resistant to 0.1 M NaOH for >100 cycles (15 min contact time)
  • Enhanced pressure/flow performance – Flows are up to 200 cm/h in a 30 x 20 cm column
  • Manufactured with reduced environmental impact

Typical Packaging

  • Bulk Resin
  • Production-Scale OPUS® Columns
  • OPUS® Robocolumns®
  • OPUS® MiniChrom Columns
  • HT Columns
Polymer Structure Highly cross linked agarose
Functional Group Protein A
Average Particle Size (d50ᵥ), μm 50 µm
Particle size range (micron) 95% between 35-90 µm
Dynamic binding capacity Up to ~60 mg hIgG/ml resin
Pressure/flow specifications Up to 200 cm/h (30 x 20 cm column)
pH stability, CIP (short term) 2 - 13
pH stability, working range 3 - 12
Recommended Storage 2 – 8°C in 20% ethanol

Purolite, An Ecolab Company