Praesto™ AP+50: Laboratory Scale Column Packing Instructions

Praesto AP+50 is a high-capacity, alkaline-stable protein A affinity chromatography resin, specifically designed for downstream processing of monoclonal antibodies and recombinant proteins. Manufactured using Ecolab's patented Jetted Technology, these resins offer uniform particle size distribution and improved flow and pressure properties, making them readily scalable for biopharmaceutical manufacturing.

This guide emphasizes the critical importance of efficient column packing to ensure reproducibility, maximize efficiency, and achieve high product yield and purity, highlighting how poor packing can lead to significant disruptions and product loss. It outlines various packing methods, including pressure/flow packing and flow/mechanical compression packing, along with procedures for slurry determination, suggested materials, and equipment.

You will also learn about essential column efficiency testing using Height Equivalent to a Theoretical Plate (HETP) and asymmetry factor (As) calculations to monitor bed deterioration. Download these instructions to learn more.