Praesto™ AP+50: Instructions For Use

This document provides comprehensive instructions for the use of Purolite Praesto™ AP+50, a high-capacity protein A resin specifically designed for purifying monoclonal antibodies (mAbs) and related constructs. It details the resin's physical and chemical characteristics, including its highly cross-linked agarose polymer structure, dynamic binding capacity, and pH stability.

The guide outlines a purification protocol, offering suggested buffers and process steps for generic mAb purification. Emphasis is placed on optimizing washing and elution steps, and the document provides insights into designing loading parameters and understanding pressure flow properties. Additionally, it covers crucial aspects such as cleaning in place (CIP) procedures , regeneration , intermediate washes , and proper storage conditions for the resin. Access these instructions and equip yourself to effectively utilize Purolite Praesto AP+50 for your purification needs.