Polyclonal (Rabbit) Anti-CaM Kinase IV

CaM kinase IV, in a Ca(2+) and calmodulin dependent manner, is implicated in phosphorylating numerous transcription factors including the CCAAT enhancer binding protein (C/EBP), serum response factor (SRF), and cyclic AMP response element binding protein (CBP). The nuclear localization of this ~55-kDa protein is consistent with its role in mediating Ca2+-dependent gene expression. CaM-KIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaM-KIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, NFAT. CaM-KIV is encoded, along with calspermin, by the Camk4 gene. It has been found that, in testes, CaM-KIV is expressed in germ cells and found to be associated with chromatin. The association of CaM-KIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.

PA1-542 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot this antibody detects an ~55 kDa protein representing CaM kinase IV (CaM-KIV) in HeLa cell lysate.

PA1-542 immunizing peptide corresponds to amino acid residues 127-143 of mouse CaM-KIV. This sequence is 94% conserved in rat and human CaM-KIV protein.

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