Physiologix™ Serum Replacement Performance On Mesenchymal Stem Cells

Mesenchymal Stem Cell (MSC) cultures are critical in regenerative medicine and cellular therapies due to their multipotent capabilities and immunosuppressive properties. Yet, traditional fetal bovine serum (FBS)-supplemented media poses several challenges, including ethical concerns, lot-to-lot variability, and potential contamination risks, which complicate regulatory compliance in cell and gene therapy (CGT) applications.

Physiologix XF is a serum- and xeno-free media supplement designed to eliminate these challenges while enhancing cell growth and maintaining critical quality attributes, such as stemness. Thie white paper details a study comparing the performance of MSCs cultured in Physiologix versus FBS, focusing on cell proliferation, viability, and pluripotency markers like CD90 expression. By reading this paper, you will learn how Physiologix outperforms FBS, particularly at 5% concentration, by accelerating cell confluence and reducing culture time by two days.

Additionally, you will learn key insights into how Physiologix enables greater lot-to-lot consistency, promoting more reliable therapeutic outcomes and facilitating CGT regulatory approval. Access the white paper below to uncover what makes Physiologix a superior alternative to FBS for MSC culture and a key step towards a safer, more efficient path for cell therapy development.