Application Note

Phenols Chloroform Extraction Protocol

Source: Thermo Fisher Scientific
GettyImages-2174865773 vial testing, lab

Phenol-chloroform extraction is a well-established laboratory technique for isolating high-quality DNA from various biological samples. This method leverages the differential solubility of DNA, proteins, and lipids in organic solvents to efficiently remove contaminants. By denaturing proteins with phenol and extracting lipids with chloroform, researchers can obtain pure DNA suitable for downstream applications such as PCR, sequencing, and cloning. While this manual technique is cost-effective, it requires careful handling of hazardous chemicals and multiple steps, making it time-consuming. For large-scale or high-throughput DNA extraction, automated methods may be more efficient.

This application note provides a detailed protocol for phenol-chloroform extraction, including the necessary materials and step-by-step instructions.

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