Pfenex Expression Technology® Platform Brief: High-Yield Microbial Expression For Scalable Biologic Production

If your late-stage biologic program depends on consistent titer, process reproducibility, and a tech transfer that reliably holds at the receiving site, the expression system you choose is vital. Pseudomonas fluorescens offers distinct advantages for recombinant protein production: periplasmic and extracellular secretion options that simplify downstream purification, inherent scalability to 180,000L, and a strain physiology that tolerates the process parameter shifts common during scale-up.

This platform brief walks you through the full workflow, from high-throughput gene-vector-strain screening and 2L fermentation scouting through research cell bank establishment, upstream and downstream process development, and analytical method co-development. Specialized capabilities include non-canonical amino acid incorporation for next-generation protein formats.

With 6 approved biologics and 25+ completed global tech transfers as reference points, you can evaluate the platform against your specific molecule class and manufacturing constraints.