Pfast Protein Expression: A Rapid, Robust Solution For Accelerating Antibody Screening

Accelerating the transition from computational design to physical protein validation requires high-throughput screening that doesn't compromise on quality. Utilizing the metabolic versatility of Pseudomonas fluorescens, researchers can now evaluate protein titer and quality in a compressed 10-day timeframe. This approach bypasses the limitations of traditional microbial systems, such as inhibitory organic acid accumulation, to achieve significantly higher biomass and soluble yields.

By leveraging combinatorial screening of genetic toolboxes, including diverse secretion leaders and host modifications, it is possible to identify optimal expression strategies for complex molecules like bispecific antibodies. These insights provide a clear roadmap for streamlining lead identification and scaling production efficiently. Watch the full presentation to see how these workflows integrate into existing biotherapeutic pipelines.