Parallel Fed-batch CHO Culture On SciVario® Twin

By Amanda Suttle and Ma Sha, Eppendorf, Inc.,

We performed fed-batch cultures using BioBLU® 1c and 3c Single-Use Vessels controlled in parallel by the new SciVario® twin bioreactor control system. This method highlights the SciVario twin’s capabilities to run complex processes simultaneously using various sized vessels. The initial release of SciVario twin can control vessel sizes from 0.7 L to 4 L. Equipped with 14 integrated advanced thermal mass-flow controllers (TMFCs) that have a turndown ratio of 1:12,000 the system is designed to be a future-proof solution. Developed following the Agile principles, future updates will allow users to run vessels from as small as 0.3 L to as large as 40 L, on the same controller. Our 3 L CHO culture reached its highest density of over 17 x 10⁶ cells per mL by day 11 with minimal human intervention. The experiments demonstrated both the capability of fed-batch cell culture and the flexibility of the  SciVario twin platform.