Parallel CHO Cell Cultivation In Eppendorf BioBLU® c Single-Use Bioreactors

By K. Blaschczok, S. Kleebank, U. Becken, and D. Eibl

In industrial bioprocess development time and cost pressure is high. A promising strategy to reduce the development time is to combine parallel experimentation with the use of single-use equipment. In this study we cooperated with Dieter Eibl and his team at Zurich University of Applied Sciences (ZHAW) to assess the reproducibility of parallel cell culture process runs in a DASbox® Mini Bioreactor System equipped with BioBLU Single-Use Vessels. We compared cell growth, viability, and the metabolic profile in four parallel bioprocesses and found that the processes developed highly similar. These results confirm the excellent suitability of this bioprocess system for efficient process development.