Optimizing DNA Clearance In High-Salt, GMP-Grade Purification Processes

Clearing host cell DNA—especially chromatin-protected fragments—remains one of the most stubborn hurdles in viral vector purification and biologics manufacturing. Traditional nuclease methods often fall short of meeting the stringent ≤10 ng residual DNA per dose requirement. This webinar introduces a next-generation salt-active endonuclease that redefines DNA clearance standards. With the ability to digest nucleic acids down to 3–5 nucleotides and maintain high activity in high-salt conditions, this technology offers a more reliable and cost-effective path to regulatory compliance. Gain insight into the limitations of conventional approaches, the advantages of deeper digestion, and how high-salt performance can improve yields and streamline manufacturing.