Optimizing DMSO Concentration And Freezing Technique For High Cell Density Cryopreservation In Upstream Bioprocessing

Many conditions must be optimized when developing a seed train for upstream processing including the medium, feed, and the starting inoculum density. When high cell density cryopreservation (HCDC) is integrated into the seed train, additional parameters must be considered. The purpose of HCDC is to intensify the upstream process, and in doing so, increase efficiency and productivity, reduce timelines, and contamination risks.

Learn more about the impact of dimethyl sulfoxide (DMSO) concentration and freezing techniques on the viable cell density (VCD) and titer production in an HCDC intensified seed train. While the HCDC process should be customized for the specific cell line being used, these results offer a starting point for developing cell line-specific conditions.