Optimize Growth: Unleash HEK Cell Potential With InstiGRO®, InstiTHAW™ And InstiSHAKE™

Gene therapy is a fascinating field that has shown promising results in the treatment of several human diseases, including tumours, autoimmune disorders, and infectious diseases. Human Embryonic Kidney 293 (HEK293) cells play a crucial role in both the research and manufacture of novel gene therapies, despite limitations in cell culture practices.

Revival and transfection often involve exposure to different environmental conditions, such as changes in temperature, osmolarity, or chemical exposure, which can stress HEK293 cell lines. Moreover, should stable transfection be the chosen route for development, the process of isolating single cells for regulatory approval can be intricate and may subject the cells to mechanical stress or other factors that can affect their health. Finally, expansion of cells for scalable manufacture subjects cells to shear forces which can affect their productivity.

Advanced Instruments proudly offers a suite of chemically defined and animal component free (ACF) cell growth supplements that supports HEK293 cells. The InstiTHAW™, InstiGRO™, and InstiSHAKE™ cell culture supplements have been carefully crafted to support the development of gene therapies, ensuring unparalleled growth and performance. InstiTHAW HEK protects cells during cryopreservation and revival, while InstiGRO HEK nourishes cells following single cell isolation, during stable cell line development. Finally, InstiSHAKE HEK supports cells in shaking culture conditions, paving the way for scalable production of recombinant viral vectors.