Application Note

Optimization Of Bacillus sacchari Fermentation And Scale Up

Optimization Of Bacillus sacchari Fermentation And Scale Up

By Bruno Sommer Ferreira and Christiane Schlottbom

Biopolymers such as polyhydroxybutyrate (PHB) are considered to be carbon-neutral, and thus environment-friendly, replacements for fossil fuel-derived plastics. They are more expensive, however, and production process costs must be reduced to increase market acceptance. Alternative feedstocks offer a promising way to reduce costs.

This application note presents the process development and optimization of wheat straw hydrolysate fermentation to produce PHB in B. sacchari. Process engineers at Biotrend® (Portugal) evaluated various ratios of two sugar concentrations on a small scale, using an Eppendorf New Brunswick™ BioFlo®/CelliGen® 115. They established automated feed triggers for the New Brunswick BioFlo 415, 610 and Pro fermentation systems, allowing them to successfully scale up the process 100-fold.

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