NS0 Serum-Free Culture And Applications

by William Whitford

Because of its adaptability, the NS0 mouse myeloma line is popular in large-scale heterologous protein expression. Industrial application has moved toward serum-free media platforms for reasons of regulatory compliance, cost, batch consistency, downstream processing, and materials availability. Large-scale serum-free NS0 culture presents some challenges. William Whitford of HyClone examines common concerns in large-scale serum-free culture and offers approaches to their resolution.

Reprinted with permission from BioProcess International V1 - No.12 pp 36-47 (December 2003)