Neuron Cell - Apoptosis Detection Kits

NeuroTACS II is a complete reagent kit optimized to provide rapid and convenient identifi-cation of apoptosis in brain tissue or neuronal cells. The kit has been developed to over-come the common difficulties unique to neuronal samples including the fragile nature of brain tissue sections, high background problems, poor counterstaining with common dyes, and the need to perform dual labeling experiments to detect cell specific antigens in con-junction with apoptotic cells. A key feature is NeuroPore, a proprietary permeabilization reagent that gently permeabilizes samples while retaining cell morphology. NeuroPore also contains blocking reagents to allow its use as an antibody diluent in immunohistochem-istry and to reduce background staining. DNA fragments generated by apoptosis are end-labeled with modified nucleotides using a highly purified terminal deoxynucleotidyl trans-ferase enzyme (TdT). The incorporated nucleotides are detected using a horseradish perox-idase system that catalyzes the conversion of diaminobenzidine (DAB) into a dark brown precipitate. NeuroTACS II contains the Blue Counterstain to allow visualization of all cells within the sample with good contrast to the brown DAB precipitate. In addition, the Blue Counterstain is compatible with the red colored substrates used for phosphatase detec-tion in double labeling experiments. The protocol includes details for labeling in situfor apoptosis and antigen detection on the same sample. To ensure that your own samples have been processed and permeabilized correctly, we provide TACS-Nuclease, a unique reagent used to generate a positive control with your own samples. This provides a high degree of confidence for data interpretation and can help pinpoint problem steps in the labeling procedure.

Features:

• Requires less than 3 hours to complete.
• Exclusive, non-toxic TACS Safe TdT buffer - sodium cacodylate free.
• Unique buffer system produces more consistent labeling.
• Performance tested on brain sections.
• Includes exclusive NeuroPore permeabilization reagent.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.

Applications:

• In situdetection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
• Assists in the identification of apoptotic morphologies.
• Helps resolve unique problems encountered when detecting apoptotic neuronal cells.

Components:

• Permeabilization reagents
• Labeling enzyme
• Chromogenic substrate
• Labeling buffer
• Optimized cation
• Counterstain
• Nucleotide mix
• Detection reagents
• TACS-Nuclease

Items not included: Reagents for dehydration and rehydration, H²O², PBS buffer, and disposables.

Related Products:

• Cell Proliferation Assays
• DNA Laddering kits
• Services and Assessories

Storage: Components are stored at -20°C, 4°C, and room temperature.

N/A, 8405 Helgerman Ct., Gaithersburg, MD 20877. Tel: 800-873-8443; Fax: 301-216-2801.