Natrix® Q Chromatography Membrane

Strong anion exchange (AEX) chromatography has become an industry standard in polishing steps for monoclonal antibody (MAb) purification. It is a proven technology to remove DNA, viruses, endotoxins and acidic host cell proteins (HCP) from process feed streams in flowthrough mode.

As the industry increasingly pursues high productivity downstream single‑use technologies and flexible biomanufacturing due to advancements in cell culture technology and the emergence of cost-sensitive biosimilars, conventional purification technologies present limitations.

Traditional, resin-based chromatography columns are often oversized due to throughput limitations and are ill‑suited to flexible manufacturing. Conventional membrane adsorbers offer faster throughput but cannot provide sufficient process robustness due to the low binding capacity. These factors impose challenges on the design of purification schemes for manufacturing of biotherapeutics.

Natrix® Q chromatography membrane overcomes these limitations by combining high binding capacity and high flow rates to deliver best-in-class performance in a single‑use, plug-and-play format.

This application note examines the performance of Natrix® Q chromatography membrane in comparison to currently available quaternary amine resins, membranes and salt-tolerant primary amine membranes. Comparative data is provided using BSA and DNA as benchmark biomolecules. In addition, the effectiveness and scalability of Natrix® Q chromatography membrane in removing process impurities (HCP, DNA & viruses) from industrial feed streams is also demonstrated.