Nanostream Veloce System Accelerates Oligonucleotide Purity Evaluation

Scientists at Nanostream, provider of high-throughput microfluidic analytical systems to companies involved in drug discovery and development, have demonstrated oligonucleotide purity assessment using the Veloce(TM) mPLC system. The Veloce system enables oligonucleotide purity assessment, a vital analysis for a number of molecular biology techniques such as cloning and ligation, 16 times faster than conventional HPLC for 384 samples. The data resulting from routine quality control (QC) of synthetic oligonucleotides using the Veloce system is within 4% of the results obtained from HPLC and demonstrates high retention time reproducibility, high efficiency and resolution with excellent peak shape.

The Nanostream Veloce system which includes instrumentation, software and 24-column microfludic Brio(TM) cartridges incorporates pressure-driven flow to achieve chromatograms comparable to HPLC for a broad class of applications while offering a dramatic increase in sample analysis capacity. The Veloce system, launched earlier this year, enables simultaneous purity assessment of 24 samples in a single run.

The oligonucleotide purity evaluation application will be presented as a poster (#14) this week at the Tides 2004 conference in Las Vegas, NV. An application note can be requested via the web site at http://www.nanostream.com/apps_appnotes.shtml or email to sales@nanostream.com.