N-Glycan Characterization Via Liquid Chromatography And Mass Spectrometry


Glycosylation is very sensitive to manufacturing parameter consistency. Any change in glycosylation can have a profound impact on an antibody’s therapeutic efficacy, pharmacokinetics/dynamics (PK/PD) and safety, so accurate monitoring and characterization is essential.

If more in-depth characterization of an mAb’s glycan profile is needed (better mass accuracy, single-glycan analysis), there are two options that require minimal additional effort to achieve information on 80%-90% of a product’s glycan population: middle-up LCMS analysis — which can identify and quantify lower-abundance glycans, main glycans, and levels of non-glycosylated product — and peptide map analysis — which can provide information on glycans, other post-translational modifications, and peptide sequence coverage, but is rarely used for glycan analysis alone.

Delve into various techniques for analyzing released glycans and their relative efficacy in HILIC-FLR-MS.

access the E-Book!

Get unlimited access to:

Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue. X

Enter your credentials below to log in. Not yet a member of Bioprocess Online? Subscribe today.

Subscribe to Bioprocess Online X

Please enter your email address and create a password to access the full content, Or log in to your account to continue.


Subscribe to Bioprocess Online

Labcorp Biopharmaceutical CMC Services