Multimeric VHH Nanobody Expression

Achieving high-expression yields of multimeric VHH antibodies requires a targeted engineering approach to balance protein titers with functional performance. By employing a specialized toolbox of secretion leaders, researchers can drive periplasmic localization to successfully produce 3X, 4X, and 5X concatemers. These complex formats are designed to significantly enhance avidity and biological half-life compared to standard single-domain units.

Utilizing high-throughput 96-well screening and refined purification methods ensures the rapid identification of optimized strains. Validated through cell-based assays, these functional multimers provide a reliable path for advancing next-generation therapeutics from early discovery into development.