mRNA Product Purification Process Strategies

Explore the mRNA universe with Cytiva. In this series, Cytiva scientists share their journey and insights on mRNA synthesis for research purposes and they share their advice on how to scale.

Impurities from in vitro transcription (IVT) – including DNA template, enzymes, NTPs, salts, double-stranded (ds) RNA, and truncated mRNA – can induce undesired immune responses and negatively impact translation efficiency. For these reasons, the mRNA must be purified. There are several options to purify mRNA: lithium chloride (LiCl) precipitation; oligo deoxythymidine (dT) magnetic particles; and chromatography. When designing a purification process, consider scalability and suitability for a manufacturing environment.

Discover the pros and cons of each option, the challenges and strategies for the sterile filtration of mRNA, and more.