Article | February 15, 2023

mRNA: Challenges And Strategies Related To The DNA Template Process

Source: Cytiva

Explore the mRNA universe with Cytiva. In this series, Cytiva scientists share their journey and insights on mRNA synthesis for research purposes and they share their advice on how to scale.

The creation of an encapsulated mRNA–LNP begins with synthesizing the DNA template. If plasmid DNA (pDNA) is used as the template, additional steps are necessary, such as purification after host cell lysis and linearization of the circular pDNA. The DNA template then undergoes purification prior to in vitro transcription (IVT), during which mRNA is formed.

Explore the DNA template, DNA template synthesis, the challenges and strategies of generating a DNA template using PCR, and more.

access the Article!

Get unlimited access to:

Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue. X

Enter your credentials below to log in. Not yet a member of Bioprocess Online? Subscribe today.

Subscribe to Bioprocess Online X

Please enter your email address and create a password to access the full content, Or log in to your account to continue.


Subscribe to Bioprocess Online