Application Note

Monoclonal Antibody Purification With A High Capacity Protein A Resin

Source: TOSOH Bioscience LLC

TOYOPEARL® AF-rProtein A HC-650F, a high capacity protein A resin for the purification of monoclonal antibodies (mAbs), was recently introduced by Tosoh Corporation. This resin, with dynamic binding capacities (DBC) of 70 g/L at 5 minutes residence time, is the newest member of the TOYOPEARL product line.

TOYOPEARL AF-rProtein A HC-650F resin remains dimensionally stable within wide extremes of pH and ionic strength. Moreover, the semi-rigid TOYOPEARL particles do not distort under flow rates that generate up to 0.3 MPa pressure. These resin properties, combined with a narrow particle size distribution, result in superior pressure-flow characteristics for the packed TOYOPEARL bed.