Monoclonal Antibody Purification: A Two-Step Approach Using A Strong AEX-HIC Mixed-Mode Resin

The rising focus on monoclonal antibodies (mAbs) in the biopharma pipeline has intensified the need for more efficient and cost-effective purification processes. Traditionally, achieving high product purity and effective viral clearance requires three distinct chromatography steps following the initial protein A capture. However, this multi-step approach introduces complexity, increases material consumption, and drives up overall production costs.

In this webinar, you will learn about an alternative process that streamlines downstream processing. By optimizing a single mixed-mode chromatography step, it is possible to eliminate an entire purification step, enabling a robust two-step strategy after protein A capture. This innovative workflow significantly cuts down on media and buffer costs, reduces processing time, and enhances the overall efficiency of your mAb production.