Application Note

Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison Of Various Mixed-Mode Media To Determine The Optimal Choice For Efficient Purification Process

Source: Bio-Rad Laboratories, Inc.

By Xuemei He

Mixed-Mode Chromatography For mAb S Aggregate Removal

In the purification of monoclonal antibodies, the separation of monomers from product-related impurities is a major challenge. Here, we highlight the importance of screening various media to determine the optimal one for an efficient purification process and illustrate that CHT Ceramic Hydroxyapatite is an effective mixed-mode chromatography media.