Mixed-Mode Chromatography Resin


Mixed-mode chromatography resin is a top tier selection for the manipulation, purification and handling of molecules; providing an unmatched level of separation properties and resolution. The combination of cation exchange resin and metal affinity resin properties, allows multimodal chromatography to provide an access route for biomolecules to separate and the use of alternative chromatographic methods make these biomolecules seem homogenous.

This selection of mixed-mode resin is appropriate for use at any stage of a given purification process, whether introduced during the initial capturing stage or used during the polishing stage of the process. Both the CHT ceramic hydroxyapatite and Bio-Gel crystalline hydroxyapatite resin have one of a kind selective, which make them ideal for various purification processes, including those done on monoclonal antibody (mAb) products.

CHT is also a multi-stage product that is rigid and spherical in nature. It is a macropourous support system made up of a combination solution of fluoroapatite and hydroxyapatite. The product is manufactured by converting hydroxyapatite nanocrystals into fluoroapatite using a chemical element. It is used to purify biologically active compounds.

Bio-Gel crystalline hydroxyapatite resin is perfect for purifying proteins as well as nucleic acids; it is also ideal for macromolecules and the product is prepared using the Tiselius method.

Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin
Nuvia cPrime hydrophobic cation exchange resin are a new addition to Bio-Rad's family of mixed-mode purification products. These resin are designed for process-scale purification of a wide variety of therapeutic proteins.

CFT™ Ceramic Fluoroapatite Resin
CFT ceramic fluoroapatite, Ca10(PO4)6F2, is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite prepared by chemically converting hydroxyapatite nanocrystals to fluoroapatite with a fluorine reagent.

Bio-Gel Hydroxyapatite HT and HTP Resin
Bio-Gel HT gel, ideal for the purification of proteins, nucleic acids, and other macromolecules, is prepared by the method of Tiselius et al., (Arch. Biochem. Biophys., 65, 132, 1956). The gel is suspended in 10 mM sodium phosphate buffer containing 0.02% NaN3 and should be stored at 4°C.