MaxCyte^® Flow Electroporation^® For Gram-Scale Transient Antibody Production In CHO-S Cells

This study evaluates MaxCyte's Flow Electroporation technology for high-titer antibody production in Chinese Hamster Ovary (CHO) cells using transient gene expression (TGE). Traditional CHO-based TGE systems have been limited by poor transfection efficiency and cell viability, leading many developers to prefer HEK-based transient systems. MaxCyte's technology can improve transfection efficiency and cell viability significantly, without requiring specific expression constructs or specialized reagents, enabling the use of CHO cells from discovery to manufacturing. The study identified several cell culture parameters that affect productivity, including cell seeding density, feeding conditions, media additives, and culture temperature. Optimization of these variables led to increased yields and reduced costs. The study also demonstrated the scalability of MaxCyte electroporation for large-scale antibody production. Using CHO cells in early development can reduce developmental delays by eliminating the need to reoptimize conditions and revalidate results when switching cell lines.