3M™ Harvest RC Single-Stage Chromatographic Purification For Recombinant Protein Therapeutic Manufacturing

The first step in the recombinant biotherapeutic process is harvesting cell culture fluid containing the product. Conventional approaches for performing this unit operation utilize a combination of depth filtration, centrifugation, and membrane filtration. These technologies utilize differences in density and in size as the principles of separation. As cell culture processes are intensified to yield higher cell densities and product titers, the ability to effectively harvest the cell culture fluid with the consistency and scalability required becomes challenging.

3M™ Harvest RC is a harvest solution that utilizes fibrous anion exchange (AEX) chromatography to efficiently separate the cells, cell debris, and DNA from the harvest fluid containing the target product. Precision quaternary ammonium (Q) functionalized polypropylene fiber, combined with a 0.2 μm PES membrane, provides scalable and predictable clarification from discovery to commercial manufacturing scale.