Liraglutide Native SEC-MALS Characterization Using Empower™ Chromatography Data System (CDS)

Understanding the true oligomeric state of peptide therapeutics requires analytical approaches that preserve native structure while delivering absolute molecular weight data. This application-focused resource explores how native size exclusion chromatography coupled with multi-angle light scattering (SEC‑MALS) can be used to characterize liraglutide under formulation‑relevant conditions. By combining SEC separation with light scattering and refractive index detection, the workflow eliminates reliance on calibration standards and avoids common sources of quantitation bias associated with UV detection—particularly for larger particulate species. The study demonstrates how careful column selection, detector choice, and integrated data processing enable accurate differentiation of higher‑order oligomers and aggregates without disrupting noncovalent assemblies.

Access the full application note to see how native SEC‑MALS supports confident peptide characterization.