Linear Scale-Up Of hiPSC Cell Lines Using Vertical-Wheel Bioreactors

Human induced pluripotent stem cells (hiPSCs) are crucial for cell and gene therapies due to their self-renewal and differentiation capabilities, bypassing ethical concerns linked to embryonic stem cells. Scaling hiPSC production, however, presents challenges in maintaining consistent cell yield and quality. This application note explores the use of Vertical-Wheel Bioreactors for linear scale-up of hiPSC cultures, from 100ml to 3L.

The study demonstrates consistent cell yield, morphology, and surface marker expression, ensuring reliable hiPSC expansion. Predictable growth rates and healthy aggregate formation highlight the bioreactors' value for process development and optimization. For detailed results and insights into how PBS Biotech can support your advancements, access the full application note.