Lentiviral Vector Analysis For Cell And Gene Therapy Made Simple

As lentiviral vectors (LVV) are increasingly used for in vivo and ex vivo gene delivery in the creation of therapies for common genetic and acquired diseases, large-scale biomanufacturing of clinical-grade LVV products must meet strict lentiviral manufacturing, product characterization, and regulatory requirements. However, there is a lack of standardized analytical assays that can meet scale-up demands to ensure safety, efficacy, quality, and lot-to-lot consistency. Typically, these assays include thorough characterization of LVV titer, stability, purity, capsid content, and transduction efficacy. To save on precious LVV sample and get to market faster, what if you could measure all these parameters with one platform?

Here, we demonstrate Simple Western’s analytical capacity to simultaneously characterize multiple parameters of LVVs, including viral titer, stability, purity, and capsid content. We show an example of Simple Western in downstream vector transduction analysis to measure LVV-mediated gene knockout in iPSC-derived macrophages.