Laboratory Scale Column Packing Instructions: DurA Cycle A50

Explore an in-depth look at the DurA Cycle A50, a protein A affinity chromatography resin used for downstream processing of monoclonal antibodies and recombinant proteins. Efficient column packing is crucial for achieving high product yield and purity. This document provides instructions for packing a laboratory-scale column using DurA Cycle A50 resin, including techniques such as pressure/flow packing and flow/mechanical compression packing. The instructions also cover adjusting the slurry percentage, calculating the required volume, and assembling the column and packing tube. Testing column efficiency is important and can be done using the height equivalent to a theoretical plate (HETP) and the asymmetry factor (As) with specific sample volume and flow velocity. The instructions also mention the importance of maintaining consistent test conditions and equipment for accurate and comparable results.