Discerning fact from fiction when freezing T cells for cell therapies.
Cryopreservation provides critical protection for cell therapies by minimizing genetic changes. But cooling too slowly or quickly risks diminishing cell viability upon thaw. On 11 March 2020, Cytiva’s Peter Kilbride (senior research scientist) and Julie Meneghel (cryobiologist) discussed the importance of controlled-rate cryopreservation. Illustrating how mammalian cells change when frozen, they offered concrete cryopreservation strategies and identified temperatures at which it is safe to stop controlled cooling and transfer drug product to cryogenic storage.