Keeping The Promise Of Immuno-Oncology With Simple Western And Single-Cell Western

Immuno-oncology has created revolutionary therapies for some, but the full promise of immuno-oncology has yet to be realized. Among the top approaches for immunotherapies are altering the tumor microenvironment to prevent immunosuppression, blocking immune checkpoint inhibitors, and reprogramming immune cells to target antigens presented on cancer cells. Despite the diversity of these approaches, a commonality is a need for protein analytical methods that preserve precious samples, while also providing assay robustness in terms of sensitivity, reproducibility, and quantification.

To realize the full potential of immuno-oncology next-generation protein analytical tools are needed to uncover the role of proteins in cancer and provide novel approaches to develop and analyze protein-based therapeutics.

Here, we show how Simple Western and Single-Cell Western have the potential to make significant contributions to three important branches of immuno-oncology: angiogenesis of the tumor microenvironment, immune checkpoint inhibition, and chimeric antigen T-cell (CAR T-cell) therapy.