Isolate Bright GFP-Expressing HEK293 Cells For Cell Line Development Workflow

Generating stable, fluorescently tagged HEK293 cell lines is a critical step in many cell biology workflows, particularly for imaging and tracking applications. This white paper outlines a streamlined approach using the Uno Single Cell Dispenser™ to isolate and expand single GFP-expressing HEK293 cells. The method achieves a 91% single-cell isolation success rate and enables selection of clones with varying GFP expression levels. Clone #10, with the highest fluorescence, demonstrated stable expression even after cryopreservation, making it ideal for long-term tracking studies. The workflow is adaptable to other genetic modifications and cell types, offering a scalable solution for researchers developing clonal cell lines.

See how this approach compares to traditional cell tracking dyes and how it can accelerate your cell line development process.