High Efficiency Separations At Any pH Or Mobile Phase

The Hamilton PRP-C18 is a new column designed to provide high efficiency reversed phase separations over an extended column life in nearly any mobile phase or pH. The rigid stationary phase has excellent mechanical and thermal stability (> 100°C), does not experience shrinkage or swelling and is completely inert to most conditions commonly encountered in reversed phase chromatography. In this study the pH stability of the PRP-C18 is evaluated. Even after prolonged exposure to concentrated (1 molar) NaOH and H2SO4 there was no measurable deterioration in performance. 

Octadecyl silane (ODS) is the prevailing stationary phase in reversed phase HPLC. Despite a well-celebrated, widespread use in chromatography, traditional ODS columns are not without limitations. Acidic conditions promote hydrolytic stripping of octadecyl functionalization, while alkaline conditions (pH > 7) attack the silica bed, both of which are principle sources for anomalous peak shape and shifting retention times that progressively worsen over the life of the column.