Intensification Of mAb Purification By Implementing Rapid Cycling Chromatography Utilizing Membrane Absorbers

By Salvador Vazquez Reyes, Grace Tan, Natalie Kay, Kady Oakley, Shawn Nie, Roy Lin, and Dogan Ornek

Column chromatography remains the standard for mAb purification, yet its limits on flow rate, residence time, and packing and qualification effort are well known, and Protein A resin rarely reaches its full lifetime in a campaign. This poster from the Process Science and Development group at the Boston Institute of Biotechnology tests a different approach: rapid cycling chromatography on membrane adsorbers, applied to an IgG4 antibody across capture and two polishing steps. It reports step yields and purity against a traditional column process, with cycle-by-cycle chromatograms and a step-level comparison of residence time, support volume, and operation time. A cost of goods model for a 30 kL GMP campaign extends the findings to production scale.

Explore the data to see how the membrane and column approaches compare, and where the authors see room for further optimization.